Network Pharmacology Strategy to Investigate the Pharmacological Mechanism of Siwu Decoction on Primary Dysmenorrhea and Molecular Docking Verification

Objective. To study the pharmacological mechanisms of Siwu decoction (SWD) on primary dysmenorrhea (PDM) and verify with molecular docking. Methods. The Traditional Chinese Medicine Systems Pharmacology Database Analysis Platform (TCMSP) was utilized to acquire active compounds their corresponding target genes. GeneCards database in search for genes that were associated PDM. intersection from SWD those PDM represented act Gene Ontology (GO) function enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses both carried out by RGUI 3.6.1 Cytoscape 3.6.0 software. also creating a compound-target network, protein-protein interaction (PPI) network created through STRING database. Molecular docking simulations macromolecular protein receptors performed using AutoDockTool 1.5.6 AutoDock Vina Results. We identified 14 as well 97 TCMSP. compared 299 related PDM, 23 which correlated 11 detected. PPI created, addition selecting most essential targets order create key network. kaempferol, beta-sitosterol, stigmasterol, myricanone. AKT1, PTGS2, ESR1, AHR, CASP3, PGR. Lastly, used confirm binding its compound. Conclusion. investigated, treating further verified.